Defense: Nanopore optofluidic devices for single molecule analysis and manipulation

Speaker Name: 
Md. Mahmudur Rahman
Speaker Title: 
Ph.D. Candidate
Start Time: 
Tuesday, November 26, 2019 - 1:00pm
Location: 
Engineering 2, Room 280

Abstract

In this talk, I will describe the development of a single molecule analysis (SMA) platform based on nanopore optofluidic chips. Using feedback control over a nanopore optofluidic device, on-demand target (70S ribosomes, λ-DNAs, Zika proteins, and NaCMC molecules) delivery is demonstrated with various reconfigurable features, including user defined number of target delivery, and automatic successive target delivery with user defined off-time. Furthermore, the feedback system can be used to selectively identify the delivery of a particular biomolecule from a mixture. Next, a novel particle trapping platform is described, which can confine particles in two dimensions to suppress the full in-plane movement. 2D particle trapping is demonstrated which manifests order of magnitude higher trap stiffness than that of a 1D trap. Finally, an elegant methodology (referred to as TACRE) is devised to improve the molecular capture rate (event frequency! ) of a nanopore. This novel technique relies on optical trapping of target carrying microbeads followed by a release of targets at the vicinity of a nanopore thus, increasing local analyte concentration. TACRE dramatically increases the nanopore capture rate due to the availability of a large number of analytes at the proximity of nanopore. Almost two orders of magnitude improvement in capture rate is demonstrated by delivering DNA targets that correspond to a melanoma cancer gene. As TACRE increases local analyte concentration, it can be implemented to detect targets at low concentrations. This is demonstrated by detecting Zika ns1 proteins down to 2ng/mL which is a clinically relevant concentration.

Event Type: 
Adancement/Defense
Advisor: 
Professor Holger Schmidt
Graduate Program: 
Electrical Engineering Ph.D.