[Genome] sequence discrepancy
Stephen Brown
sabrown at uvm.edu
Fri Feb 1 09:04:23 PST 2008
Dear All:
I have found a rat gene (GAJ1) where electronic PCR on the UCSC browser
yields a product length (with the primers i've chosen) of 429 bases.
PCR from the cDNA/mRNA yields a predicted length of 498. i have not
yet done the PCR to see what the truth is, but i assume it will be
498.
primers in question are:
CTGCGAAAACGTCTGCTATG
GGACGTGAGAGGAAGCAGTC
its really not that important, but i wondered if you (pl) would have
insight into what happened to the ~70 bases??
thanks
steve brown
University of Vermont
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