[Genome] missing data on in silico pcr

[Galt Barber]

We are not aware of any changes that would create this situation.

Perhaps you would like to send us an example to try of one that
you believe used to work, along with relevant details
such as species, assembly, primers, and the results
you got before?  Can you clarify that you used the
hgPcr cgi on our website, or did you download and
run your own standalone isPcr?

-Galt


On Sun, 20 Apr 2008, Rick Scavetta wrote:

> Hello,
>
> About a year ago I tested some primers on in silico PCR and found about
> 42 regions in the mouse reference genome at the time (that's about what
> I was expecting). However, I wanted to go back and double check, but
> when I do the same thing now, I don't find anything. I have tried using
> the latest build and also older ones which were active when I did the
> original query. I also lowered the min good match, but that didn't seem
> to help.
>
> Do you have any idea why I don't get any results anymore?
>
> Thanks,
>
> Rick Scavetta
>
> --
> Rick J Scavetta
> Max-Planck-Institut fuer Evolutionsbiologie
> Abteilung Evolutionsgenetik
> August-Thienemannstrasse 2
> 24306 Ploen (Germany)
> Tel.: 04522 763 279
> scavetta at evolbio.mpg.de
>
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