[Genome] WT1 gene clone

[Robert Kuhn]

Dear Dr. Brown,

I agree with your interpretation, as the first two short
alignment blocks are essentially poly-(CAT) and encode
polyhistidine, according to the genbank record from 1995.

However, our display of genbank records is an automated process 
and we do not do any manual curation of the mRNA alignments.
The only way to remove this accession from the browser is
for the original contributors of the sequence to remove the
record from genbank, though perhaps the genbank staff could
change the DEFINITION field to reflect the poly-His tag (see
REMARK field below).  The latter solution would still not remove
the alignment from the mRNA track in the browser, however,

The 3' end of the sequence does have a good alignment to this 
region.  As with any database resource of biological data, the 
user needs to use his/her own judgement, as you have done here, 
to determine how best to proceed in the interpretation of data.   

best wishes,
				--b0b kuhn
				ucsc genome bioinformatics group


LOCUS       S75264                   521 bp    mRNA    linear   PRI 11-JUL-1995
DEFINITION  WT1=Wilms' tumor suppressor protein [human, fetal kidney, mRNA, 521
            nt].
ACCESSION   S75264
VERSION     S75264.1  GI:896246
KEYWORDS    .
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Hamilton,T.B., Barilla,K.C. and Romaniuk,P.J.
  TITLE     High affinity binding sites for the Wilms' tumour suppressor
            protein WT1
  JOURNAL   Nucleic Acids Res. 23 (2), 277-284 (1995)
   PUBMED   7862533
  REMARK    GenBank staff at the National Library of Medicine created this
            entry [NCBI gibbsq 160293] from the original journal article.



> From genome-bounces at soe.ucsc.edu  Fri Sep  7 09:08:32 2007
> To: genome at soe.ucsc.edu
> Subject: [Genome] WT1 gene clone
> 
> Hi,
> Just been looking at the human WT1 gene (chr11:32,366,412-32,471,435) for 
> longer transcripts in UCSC Genome Browser and found S75264, which at first 
> site looks very interesting (listed under "Human mRNAs from Genbank). 
> However, looking up the reference, it is clear that this is the sequence of 
> a polyhistidine tagged recombinant clone.  Could you get this removed from 
> the next human assembly to avoid further confusion?
> Many thanks,
> Keith Brown
> ----------------------
> Dr Keith Brown
> Reader in Molecular Pathology
> University of Bristol
> Department of Cellular & Molecular Medicine
> School of Medical Sciences
> University Walk
> Bristol BS8 1TD
> UK
> Tel:+44 (0)117 3312071
> Fax:+44 (0)117 9287896
> EMail:Keith.Brown at bristol.ac.uk
> 
> 
> 
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