[Genome] Question
Archana Thakkapallayil
archanat at soe.ucsc.edu
Tue Sep 4 14:35:04 PDT 2007
Hello Chen,
This task can be accomplished with the Table Browser intersection tool.
It will involve first creating a custom track with your BED file. In
order to get the list of genes that are within 1kb distance to your
regions, first take the BED file and then subtract 1,000 from all the
starts and add 1,000 to all the ends (so that all of the chrom regions
are now 1,000 bases longer on either side). Then upload that BED file as
a custom track.
Please see the following link for information on creating custom
annotation tracks:
http://www.genome.ucsc.edu/goldenPath/help/hgTracksHelp.html#CustomTracks
Then intersect your custom track with the 'UCSC Genes' or the 'RefSeq
Genes' track using the Table Browser intersection tool. More information
on using the intersection tool is here:
http://www.genome.ucsc.edu/goldenPath/help/hgTablesHelp.html#Intersection
Note that when you do a Table Browser intersection, the results only
give the items and their positions from the first table that you
selected. So to obtain the list of gene names, you'll have to select the
gene track as the first track, and your custom track as the second track
for the intersection.
I hope that this helps. If you have further questions please don't
hesitate to contact us.
Regards,
Archana
UCSC Genome Bioinformatics Group
Chen Su wrote:
> Hi,
> I have a quick question. I have a BED file with chrom regions. and I'd
> like to know the list of genes that either overlap or are within 1kb
> distance to those regions. What's the best way to do this? Thanks a lot
> for your help!
> - Chen
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> Genome maillist - Genome at soe.ucsc.edu
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