[Genome] Question on multiz

Tay Sen Kwan taysk at novasprint.com
Tue Oct 9 21:23:12 PDT 2007


I am using the Genome Browser to look at the conservation of certain 
cDNAs across a number of species, in particular the 28-species multiz 
conservation track.  For example, BC069077 which lies on chr15 q11.2, 
showed sequence alignment with the rabbit.  However, when I extracted 
the rabbit sequence from the rabbit track, remove the gaps "-" and did 3 
alignment tests (with other tools) on it:
(a) a "Align two sequences using BLAST (bl2seq)"  with BC069077 on NCBI 
Blast  - it gave a "no significant similarity" result
(b) a NCBI BLASTN on the human sequence with a very relaxed Expect=10 
value, and it found no alignments on chr15
(c) a BLAT which also turn up no alignments on chr15

None of these tests indicated alignment to BC069077.  Could you help me 
resolve this apparent contradiction ?  Many thanks.

Regards,

Sen Kwan


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