[Genome] Question on multiz
Tay Sen Kwan
taysk at novasprint.com
Tue Oct 9 21:23:12 PDT 2007
I am using the Genome Browser to look at the conservation of certain
cDNAs across a number of species, in particular the 28-species multiz
conservation track. For example, BC069077 which lies on chr15 q11.2,
showed sequence alignment with the rabbit. However, when I extracted
the rabbit sequence from the rabbit track, remove the gaps "-" and did 3
alignment tests (with other tools) on it:
(a) a "Align two sequences using BLAST (bl2seq)" with BC069077 on NCBI
Blast - it gave a "no significant similarity" result
(b) a NCBI BLASTN on the human sequence with a very relaxed Expect=10
value, and it found no alignments on chr15
(c) a BLAT which also turn up no alignments on chr15
None of these tests indicated alignment to BC069077. Could you help me
resolve this apparent contradiction ? Many thanks.
Regards,
Sen Kwan
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