[Genome] how to identify transcription factors

Irfan Qureshi oldandbusted at gmail.com
Mon Mar 19 14:45:26 PDT 2007


Hi everyone-
I'm wondering if I'm doing this right or if there is a better way to  
identify the TF associated with a given gene.
Thanks


Using the Table Browser

1.  select the following
Group: known gene
Track: known gene
Identifier- enter the gene of interest (ie NM_X)
Output- custom track
whole gene or upstream ? (I'm unsure what to select here)

2.  select the following
Group: expression and regulation
Track: tfbs
Table cons_sites
Intersect with custom track from 1
Output: custom track
whole gene? (I'm also unsure what to select here)

3.  select the following
Group: custom track
Track: select custom track from 2
clear intersection
Output get selected fields- name of tfbs (ie V$X)

4.  select the following
Group expression and regulation
Track: tfbs
Table: cons_factors
Filter: enter names of tfbs from 3
Output: selected fields from table
choose "actors (ie myc)




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