[Genome] Clone question

[Ann Zweig]

Hello Julie,

	We map the chromosome bands with data we get from NCBI and the FISH 
data, in this case, from Fred Hutchinson and Wellcome Trust.  All 
cytology is subject to the low resolution of light microscopy, and 
either value could be off.  The sequence from the NCBI record definitely 
BLATs to the sequence location indicated in the browser.

	Note that the chromosome bands were generated about one year ago -- 
when we were creating the hg18 browser.  The FISH data were deposited 
into NCBI in 1999.


Regards,

----------
Ann Zweig
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu



Gorenstein, Julie wrote:
> Hi,
> For this FISH clone, the band position is 10q26.13, but the FISH
> Placements say that it's 10q23.1.  Could you please clarify the
> difference?
> 
> Thank you,
> Julie
> 
> 
> RP11-62L18					
> 					
> 	RP11-62L18
> <http://www.ncbi.nlm.nih.gov/genome/clone/clname.cgi?stype=Name&list=RP1
> 1-62L18> 
> 				
> 	Chromosome:	chr10			
> 	Start:	123214091			
> 	End:	123388488			
> 	Band:	10q26.13			
> 	
> Placement of RP11-62L18 on draft sequence was determined using the
> location of Accession				
> 	Genbank Accession:	AC009988.12
> <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&
> term=AC009988.12&doptcmdl=GenBank&tool=genome.ucsc.edu>
> 
> 	BAC end sequence:	AQ195323
> <http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Search&db=Nucleotide&
> term=AQ195323&doptcmdl=GenBank&tool=genome.ucsc.edu> 			
> 
> 					
> 
> FISH Placements 		
> 		
> 	Lab	Band Position		
> 	SC	10q23.1 - 10q23.33		
> 	FHCRC	10q23.1 - 10q23.33		
> 
> 
> 
> 
> 
> 
> Julie Gorenstein
> Target Validation
> Merck Research Laboratories -- Boston
> BMB 9-107
> 33 Avenue Louis Pasteur
> Boston, MA 02115
> (617) 992-2548
> julie_gorenstein at merck.com
> 
> 
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