[Genome] Use of ENCODE project data
Ann Zweig
ann at soe.ucsc.edu
Wed Jan 10 16:26:35 PST 2007
Hello Philip,
I would suggest you start at our ENCODE portal:
http://genome.ucsc.edu/ENCODE/ From here you will have access to all
of the published ENCODE data.
- You can view these data in the genome browser:
http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg17&position=chr7:115,404,472-117,281,897&encodeRegions=full
- You can download ENCODE data tables:
http://hgdownload.cse.ucsc.edu/goldenPath/encode/index.html
- You can perform queries on the ENCODE data tables:
http://genome.ucsc.edu/cgi-bin/hgTables
There is ENCODE data available on two human assemblies: May 2004 (UCSC
hg17, NCBI Build 35), and Jul. 2003 (UCSC hg16, NCBI Build 34).
To become familiar with the data that are available, start with the
genome browser. Navigate to the hg17 assembly and scroll down the page
to the track controls until you start to see the ENCODE sections. For
example, when you get to the section entitled "ENCODE Transcript
Levels", you will see the following tracks:
Affy RNA Signal
Affy Transfrags
BU First Exon
Riken CAGE
Stanf Promoter
Stanf RTPCR
Yale MAS RNA
Yale MAS TAR
Yale RNA
Yale TAR
Change the visibility of the tracks to see them in the display. Or,
click on the track name to link to a description page that gives many
details about the track, including Methods, Verification, Credits, and
References.
Once you become familiar with the available data, I think it will
become clear to you how to find the functional elements you are looking for.
Regards,
----------
Ann Zweig
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu
Philip M Terry wrote:
> Hello,
>
> May I ask the following question concerning the ENCODE project:
>
> I am a novice with respect the the ENCODE project. I would like to
> find/locate (in the human genome) functional elements such as transcription
> binding site locations, promoter regions, nucleosome poor regions, etc. Can
> you suggest how I should proceed?
>
> Sincerely,
> Philip M. Terry, PhD
> University of Nebraska-Lincoln
>
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