[Genome] please help

[Andy McCallion]

Hi Jim (and Co.)
I have a question for you and I think we may need your help in 
resolving it. As you may remember one of the project underway in my 
lab involves tiling across the zebrafish phox2b interval. I have a 
young student who has now  evaluated every amplicon by transgenesis 
in zebrafish. The data look really cool but now as we pursue the post 
hoc bioinformatics to evaluate what regulatory elements are 
identified and what are missed by established algorithms we have 
discovered a problem with the  most recent build of the zebrafish 
(2006) - the locus has been inappropriately duplicated within the 
same chromosome. The people at Sanger are now aware of this but are 
not making speedy progress on remedying  it. The 2005 build looks 
great but it does not have the benefit of multiZ and phastcons tracks 
that incorporate the orthologous opossum and xenopus sequences etc. I 
would really like to be able to view this interval in light of this 
additional information. Is there any convenient way to get these 
tracks generated or to have them placed on the 2005 build?

Thanks in anticipation of your help with this
Cheers
Andy

-- 
-----------------------------------------------
Andrew S. McCallion Ph.D.
Assistant Professor
McKusick-Nathans Institute for Genetic Medicine
Johns Hopkins University School of Medicine
BRB Room 449
733 N. Broadway
Baltimore, MD, 21205
USA

Tel:  (443)-287-5624 (Office)
Tel:  (410)-502-7533 (Lab)
Fax:  (410)-614-8600
email:  amccalli at jhmi.edu
Gen Med: http://www.hopkinsmedicine.org/geneticmedicine/
Comp Med: http://www.hopkinsmedicine.org/comparativemedicine/


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