[Genome] BLAT against mm9.2bit

Sukhinder Sandhu sukhinder.sandhu at osumc.edu
Fri Aug 31 12:31:17 PDT 2007


Hi

I can do small BLAts but when I try to BLAT the same file against the whole
genome build, I get the following error:

[CANCER-W8706199WOH:~/Desktop/usr/blatSuite] sand60% ./blat /Volumes/MAC\
USERS\$/sand60/mm9.2bit
/Users/sand60/Desktop/FRA_LOH_DEl/humanMirs_known.fasta ./aug31_try1.psl
Loaded 2725765481 letters in 35 sequences
blat(627) malloc: *** vm_allocate(size=879542272) failed (error code=3)
blat(627) malloc: *** error: can't allocate region
blat(627) malloc: *** set a breakpoint in szone_error to debug
needHugeMen: Out of huge memory - request size 879541876 bytes

What would be the best way around this? Any suggestions are greatly
appreciated. Thanks a lot.

sukhinder



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