[Genome] Blat 35-mer perfect matches

Derek Chiang dchiang at broad.mit.edu
Wed Aug 15 18:52:33 PDT 2007


What parameter settings on the standalone blat would you recommend to 
find perfect sequence matches of 35 bases in the human genome?  I've 
tried the parameter settings of -minMatch=3 -minIdentity=100 but noticed 
that some query sequences lacked any hits.  I assume that tiles with low 
complexity are filtered out, thus failing the -minMatch requirement.

Thanks,
Derek


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