[Genome] Browser shows complement, unexpectedly?
Thomas D. Moloshok
Thomas.Moloshok at fccc.edu
Wed Apr 25 08:33:33 PDT 2007
Hi,
Yesterday I was looking for the potential stop codon of Mouse (mm8) Pax1
Chr2:147056501-147066487
Having downloaded the sequence using the DNA link of the browser
("5'pad=0 3'pad=0 revComp=FALSE strand=? repeatMasking=none"),
I found that one is ATTAAA
Chr2:147066098-147066103
Viewing this location in the browser shows:
TAATTT
i.e., the complement!
When I looked at the stop codon of the known gene: TAG
Chr2:147066098-147066103
Again, I see the complement: ATC
which would actually code for Ile.
These are not the reverse-complement, and Pax1 is listed as reading
on the + strand.
Any suggestion of what I am doing, or is going, wrong?
Thanks,
Tom
<<<o>
Thomas Moloshok, Ph.D.
Director, Bioinformatics
Fox Chase Cancer Center
333 Cottman Avenue
Philadelphia, PA 19111-2497
215-214-1417
More information about the Genome
mailing list