[Genome] direction of ESTs

[ericho@eden.rutgers.edu]

Hi Helpdesk,

I've a question about the meaning of arrowheads displayed on the EST tracks.

E.g. in Human Mar 2006 Assembly, region chr11:46,707,502-46,707,591, with
both spliced and unspliced human ESTS set to 'full'.

many ESTs are displayed under the gene.

I noticed that some ESTs have arrowheads '>>>>>' or '<<<<<<' on them, I
think '>>>>>' map to + and '<<<<<<' map to - strand. But it is not.

e.g. If you click on EST CB156997 with '>>>>>', it says it is aligned to
'-' strand in chromosome 11. Ok, may be the arrowhead represents in the
opposite way i.e. '>>>>>' means - strand, '<<<<<<' means + strand.

However, when I click on EST AI133113 which has '<<<<<' on it, the record
also say it is mapped to '-' strand too. Then I'm confused.

Could you tell me in what way I should interpret the arrowheads? Also,
many ESTs don't have arrowheads on it, what does that mean?


Thanks,
-- Eric