[Genome] gene alignment
Krista R Casazza
kristac at uab.edu
Thu Nov 30 06:54:47 PST 2006
Hi
I am a postdoc at UAB and we are looking at a gene in drosophila LanA on
chromosome 3L that is involved in fat storage. An ortholog Lama5 is on
chromosome 20 in humans. I am trying to determine if there is alignment
or conservation between the 2 genes. I got the fly sequence from
ensembl and the human sequence from Entrez SNP and ran it through BLAT
but got no hits. It was suggested that BLAT may not be sensitive enough
to pick up human/fly orthologs, as BLAT, for the most part, was written
to map mRNAs and traces back to
the genome, or a closely related genome, very quickly. It was also
suggested that I probably want to do the alignments with a program like
blastp. I have attached the text format of the genes. Is it possible
for you to help me with this alignment. As you can probably ascertain,
my PhD is not in genetics and this research is very new to me. I would
appreciate any assistance that you may have to offer.
Thanks
Krista
Krista Casazza, PhD, RD
Postdoctoral Fellow
University of Alabama at Birmingham
WEBB 415
1675 University Blvd.
Birmingham, AL 35294
(205) 975-6226
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