[Genome] Batch BLATs

Donna Karolchik donnak at soe.ucsc.edu
Tue Nov 7 10:19:34 PST 2006


hi Bob,

Program-driven use of web-based blat is limited to a maximum of one hit every 15 
seconds and no more than 5,000 hits per day. As you have discovered, we have 
automated processed in place to slow down or halt access that exceeds these 
limits. This prevents one user from monopolizing our server or (in extreme 
cases) bringing the server down.

If it's feasible, I suggest you install a local copy of blat, which will allow 
you efficient unlimited access. For more information on downloading and 
installing blat, see our FAQ: http://genome.ucsc.edu/FAQ/FAQblat#blat3. A lot of 
individuals at your institution use our software, so I suspect there are local 
blat servers available. If you ask around, you may be able to find one, if you'd 
prefer to not install it yourself.

-Donna
-----------------------------------
Donna Karolchik
UCSC Genome Bioinformatics Group
http://genome.ucsc.edu

----- Original Message ----- 
From: "Robert Palermo" <palermor at u.washington.edu>
To: <genome at cse.ucsc.edu>
Cc: <genome-www at cse.ucsc.edu>
Sent: Monday, November 06, 2006 11:26 PM
Subject: [Genome] Batch BLATs


>I have been running a large number of BLAT jobs in interactive mode,
> aligning about 500 sequence elements against the Rhesus Genome.
>
> I have been getting notices that this amount of traffic is now
> getting penalized.
>
> This is a chore I am running in interactive mode tonight, doing in
> batches of 25 sequences.  If you can give me a faster, tractable
> route for this one-time job, please let me know.
>
> Bob Palermo
>
>
> _______________________________________________
> Genome maillist  -  Genome at soe.ucsc.edu
> http://www.soe.ucsc.edu/mailman/listinfo/genome
> 



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