[Genome] Rat Ensembl genes and QTLs
Archana Thakkapallayil
archanat at soe.ucsc.edu
Fri Nov 3 10:17:58 PST 2006
Hello Rick,
This task can be accomplished with the Table Browser intersection tool.
Click the "Tables" link at the top of the page and follow these steps:
1. Select the genome and assembly of interest and then choose 'group:
Genes and Gene Prediction Tracks', 'track: Ensembl Genes', and 'table:
ensGene'.
2. Select "genome" as the region.
3. Click on the "paste list" or "upload list" button to add the list of
Ensembl gene ids. You need to have a list of your Ensembl gene ids in a
consistent format that the Table Browser can use (one ID per line).
4. Select custom track as the output and press the "get output" button.
Then hit "get custom track in table browser" button. This creates a
track in the Table Browser that represents the Ensembl genes that you
have uploaded.
5. Then you can create an intersection of these genes with the RGD QTL
track. To do this make the following selections:
group: Mapping and Sequencing Tracks
track: RGD QTL
table: rgdQtl
region: genome
Press the "intersection: create" button and on the intersection page
choose 'Custom Tracks' as the group name, and the custom track and table
name. Then select the radio button 'All RGD QTL records that have any
overlap with tb_ensGene' and hit "submit".
6. Output the intersection as BED or custom track. This gives you the
list of all QTL's that overlaps with your Ensembl gene list.
I hope that this helps you. Please let us know if you have further
questions.
Regards,
Archana
UCSC Genome Bioinformatics Group
Dixon, Dr R. wrote:
> Dear Genome team,
>
> I have a list of Ensembl Gene ids (hundreds) (ENSG....) - I would like
> to obtain the QTLs that overlap with these genes... How do I do this
> with the ucsc browser table structre
>
> Thankyou
>
> Rick
>
>
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