[Genome] Asking for help from China

lxq coastalee at ion.ac.cn
Wed Dec 20 08:27:16 PST 2006


Dear Genome,
     This is Coasta from the Institute of Neuroscience, Chinese Academy of Sciences. I have fallen into some problems handling zebrafish gene sequence. One of my interested genes, that is GFAP cDNA, is sequenced using the Singapore local strain. I have streaked out several clones after ligation for sequencing. Compared with the published sequence, there are four common mutations among them. So I suspected there are discrepancies among different fish strains. And I¡¯m just wondering what the identity of the Singapore local strain is. Are they the AB line or Tuebingen Line? Thanks for your kindly help,
    Best,
Sincerely,
Yours,
Coasta


Lab of Sensory Integration and behavior
Institute of Neuroscience
Chinese Academy of Sciences
320 Yueyang Road 
Shanghai£¬200031
P.R. China

Tel: (86)-21-5492-1707



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