[Genome] are the "minus" strand in the "gete data" results from the galaxy browser reverse-complimented?

Dominik Margraf dominik.margraf at gmail.com
Mon Dec 4 13:46:36 PST 2006


I am performing searches using the "get data" function from the Galaxy
browser (the known gene track) and the results are returned in BED
format, which I subsequently used to obtain the actual sequences in
FASTA format.

However for the sequences in the "minus" strand, are these sequences
(in FASTA file) already reverse-complimented to the "plus strand"?  If
so, then would it be okay to directly use the BED file to intersect
with other tracks (e.g. the phastcons17way track / BED) which do not
have strand information?

Thanks!

Dominik


More information about the Genome mailing list